Amelie Wamba Ndongmo Regine

PhD student, Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon.

Supervisor : Pr. Jude D. BIGOGA

PhD student Intern, Centre for Research in Infectious Diseases (CRID), Liverpool School of Tropical Medicine (LSTM-unit), Yaounde, cameroon.

Supervisor: Pr. Charles S. WONDJI

• PhD research-title: Investigating the role of overexpressed cytochrome P450 CYP325A in pyrethroid-based metabolic resistance in Anopheles funestus, a major malaria vector across Africa.
• Research objectives involved using molecular biology techniques (PCR, cloning and sequencing) to amplify and characterize a mosquito P450 gene (CYP325A) supervised by Dr Michael O. Kusimo.
• In silico predictions (modelling and molecular docking simulations) using Modeller and Molegro Virtual Docker software.
• In vitro ( heterologous expression in pCW vector of P450 in E. coli coexpressed with CPR, metabolism assays with insecticides and enzyme turnover kinetic studies) This par was conducted in the Vector Biology Laboratory of Pr. Charles Wondji supervised by Dr Sulaiman S. Ibrahim, at the Liverpool School of Tropical Medicine, United Kingdom.
• In vivo techniques of RNA interference and transgenic expression of mosquito gene in Drosphila melanogaster flies (pUAS-Gal4 system)
• Research duties and work were supported by a Wellcome Trust Senior Research Fellowship in Biomedical Sciences to CSW (101893/Z/13/Z and 217188/Z/19/Z) as a scholarship student.

Supervisor: Pr. Jude D. BIGOGA, Molecular Parasitology and Disease Vector Research Laboratory, The Biotechnology Centre (BTC), Nkolbisson, Yaounde, Cameroon.

Department of Biochemistry, Faculty of Science, University of Yaounde I, Cameroon.

Masters research title: Investigating the larvicidal potential of Tithonia diversifolia , a sunflower plant against Anopheles and Culicine mosquitoes in Yaounde, Cameroon.

Research duties involved:

• Mandatory course work at the university and a minimum of 2.4GPA for the masters one level.
• Collection of sunflower leaves and flowers and pre-treatment before extraction procedures.
• Hydrodistillation of fresh samples to acquire essential oils for HPLC analyses of composition.
• Cold aqueous and ethanol maceration of dried samples and performed phytochemical screening of secondary metabolites on macerates
• Field collection of mosquitoes from larval breeding sites and rearing in the laboratory to stage 4 for bioassay.
• Conducted larvicidal bioassay on Anopeles and culicine larvae and registered susceptibility levels through mortality rates.

Supervisor: Mr Kevin Keller, Associate at IDinsight, 7 Offices, Globally, United States

• Managed team of team of over 5 people in Northern and Adamawa regions in Cameroon overseeing collection of prospective and retrospective data on neonatal healthcare in regional hospitals.
• Conducted meetings with administrative, local and hospital authorities to collect information on quality of care and explain importance of KMC project (Kangaroo Mother Care) in improving neonatal care and survival rate for pre-term babies.
• Worked as English/French translator at meeting and for documentation to facilitate communication between stakeholders and Cameroon government officials mostly french-speaking.

Supervisor: Mr. Wounang S. Romain, Economics and Statistics Specialist, NIS.

• Responsible for pilot data collection using electronic questionnaires on tobacco consumption habits of population
• Translation of electronic questionnaires from English to French because 80% of Cameroon is french-speaking.
• Performed duties in accordance with all applicable standards, policies and regulatory guidelines to promote safe working environment for the correction and verification of questionnaires after data collection.
• Developed and implemented performance improvement strategies for assessment of questionnaire clarity.

Supervisor: Mr Njiki H. Murray, Economics and Statistics Specialist, NIS.

• Responsible for team of 6 people (01 driver and 05 field workers) on collection of data on use of mosquito nets and prevention of malaria
• Established meetings with administrative and local authorities on behalf of the Ministry of public relating to study in question for smooth running of data collection and team safety
• Engaged the local populations to actively participate in the study.
• Sensitized locals on the proper use of bed nets and importance in vector control against malaria.
• Ensured correction and transmission of questionnaires to headquarters as well as planning, organizing work and meeting deadlines.

Supervisor: Mr Wounang S. Romain, Economics and Statistics Specialist, NIS.

• Responsible for team of 6 people (01 driver and 05 field workers) on collection of data on access to potable water and sanitation in rural areas
• Established meetings with administrative and local authorities on behalf of the Ministry of public relating to study in question for smooth running of data collection and team safety
• Engaged the local populations to actively participate in the study.
• Sensitized locals on the importance of potable water and how to make available water safe for drinking and cooking.
• Ensured correction and transmission of questionnaires to headquarters as well as planning, organizing work and meeting deadlines.

Supervisors: Mr Wounang S. Romain and Mr Sango Pierre, Economics and Statistics Specialists, NIS.

• Part of team of 6 people (01 driver and 05 field workers) on collection of data on health and demographics in local communities.
• Administered questionnaires in French and English to collect data on health and demographics per selected household.
• Collected blood samples for the performance of Rapid Diagnostic Tests (RDTs) for malaria and anemia and storage for subsequent HIV tests.
• Collected anthropometric data such as temperature, height, weight and blood pressure using provided equipment.
• Ensure correction and transmission of questionnaires to team leader as well as meeting deadlines.

Supervisors: Sales Managers of Supermarket

• Internship as sales assistant in two renowned supermarkets; DOVV and CASINO in Yaoundé.
• Responsibilities included customer service and inventory control.
• Served over 50 customers per day and increased sales by 15%.
• Endorsed advising and assisting clients, dealing with unexpected problems and situations.
• Volunteered for extra shifts during holidays and other busy periods to alleviate staffing shortages.
• Stocked merchandise, clearly labeling items, and arranging according to size or color.
• Worked closely with shift manager to solve problems and handle customer concerns.